6). In Fig. 6 (E-SAL), epithelial and endothelial cells were not present. BMDMC attenuated all these ultrastructural changes and yielded multinucleated cells and type II pneumocytes with large hyperplasia of lamellar bodies. Additionally, epithelial and endothelial cells were noted around the interstitium. Cell therapy also mitigated airway damage, reducing basement membrane irregularity and fragmentation, as well as disorganization and detachment of the airway epithelial cell layer (Table

3, Fig. 6). Y chromosome DNA was not detected in lung tissue at 5 weeks in cell-treated groups. TGF-β, PDGF, and IGF mRNA expressions in lung tissue were higher, while VEGF expression was lower in E-SAL compared to C-SAL. BMDMC administration led to an increase in IGF and VEGF mRNA expressions, and a reduction in TGF-β and PDGF expressions KRX-0401 nmr Selleck Cilengitide (Fig. 7). In the present murine model of pulmonary elatase-induced emphysema, early intravenous BMDMC therapy led to: (1) reduction in mean linear intercept, fraction area of alveolar collapse and hyperinflation; (2) reduction in

the number of mononuclear cells and neutrophils and collagen fiber deposition in lung tissue; (3) increase in elastic fibers in the alveolar septa; (4) decrease in airway epithelium and alveolar-capillary membrane damage as well as elastic fiber breakdown; (5) reduction in the degree of lung apoptotic cells and caspase-3 expression, and (6) improvement of right ventricular wall thickness and right ventricular Amrubicin area followed by a reduction in collagen fibers in lung arterial vessels. Our findings suggest that the lung and heart may be protected by a mechanism linked to a balance between growth factors. In the present study, emphysema was induced by multiple intratracheal instillations of porcine pancreatic elastase. This model, initially described in NMRI

mice (Luthje et al., 2009), leads to lung, cardiovascular, and systemic impairment (Antunes and Rocco, 2011), and induced lung and cardiovascular damage in our C57BL/6 mice. Moreover, this method of emphysema induction yields lengthy progressive inflammatory and remodeling processes, in addition to alveolar destruction. The present study is the first to demonstrate the protective effects of early BMDMC administration to the lung and heart in experimental pulmonary elastase-induced emphysema. To identify this homing of bone marrow cells to the lung parenchyma, we used PCR of Y chromosome-specific sequences. However, Y chromosome DNA was not detected in lung tissue at day 28 in cell-treated groups, suggesting that the benefits we observed probably result from paracrine effects.