infantum challenge as illustrated by a dramatic decrease in paras

infantum challenge as illustrated by a dramatic decrease in parasite burden both in the liver and in the spleen of immunized mice at 4 weeks following challenge. At this time point after infectious challenge, mice vaccinated with G1 and G2 demonstrated significantly lower amount of parasite load in both liver and spleen and a clear correlation between IFN-γ :IL-10 ratio upon stimulation with F/T L. infantum, and parasite burden in liver [−0·847** (P = 0·008)] and spleen [−0·699 (P = 0·054)] was observed. This correlation is in concordance

with histopathological findings as no parasites were detected in the liver and spleen of G1 and G2 4 weeks after challenge, whereas they were easily seen in the tissues of G3 and G4. Interestingly, at 12 weeks after challenge, G1 and G2 showed Dinaciclib manufacturer lower parasite propagation in the spleen than control groups (G3 and G4) due to decreasing parasite burden slope

between weeks 8 and 12 in vaccinated groups. Vaccination with the pcDNA–A2–CPA–CPB−CTE before and after infection was associated with the production of specific IgG1 and IgG2a antibodies against the rA2–rCPA–rCPB and F/T L. infantum antigens, with IgG2a-specific antibodies being induced before IgG1 antibodies. Thus, these data indicate that DNA vaccination delivered either by physical or by chemical route induced specific Th1 and Th2 cells, with Th1 cells being generated first. Immunity to L. infantum is associated with the preferential Adenosine triphosphate induction of a Th1 response, but Th2 responses have also been shown to be important in conferring protection [42]. Nitric oxide (NO) production by the inducible iNOS (or NOS2) synthase represents one of the main microbicidal mechanisms of murine macrophages and can be regarded as a natural antiprotozoan weapon [43]. According to Brandonisio et al. [44], protection against leishmaniasis is associated with increased expression of iNOS and higher levels of NO. In this report, we showed that DNA vaccination with pcDNA–A2–CPA–CPB−CTE

induces considerably appropriate humoral and cellular immune responses in addition to NO2 generation upon rA2–rCPA–rCPB- and F/T L. infantum-specific stimulation, 8 weeks after infectious challenge with L. infantum. Although G1 vaccinated via electroporation shows a higher amount of rA2–rCPA–rCPB- and F/T L. infantum-specific NO2 production than G2 with cSLN formulation, there are significant differences between G2 and control groups. Also a major factor contributing to healing in leishmaniasis is the development of strong cell-mediated immunity (CMI) responses like IFN-γ and NO production [45-47]. Therefore, higher amount of IFN-γ and NO2 production in G1 and G2 in comparison with the control groups represents a fine correlation between CMI and resistance to infection.

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