(XLS 149 KB) Additional file 2: Full list and taxonomy of OTUs clustered at 6% difference in descending order of their relative abundance (%). This is an Excel file listing all 517 OTUs, abundance and the taxonomic assignment of each OTU per individual S1, Selleck EPZ5676 S2 and S3. (XLS 116 KB) Additional file 3: Full

list and taxonomy of OTUs clustered at 10% difference in descending order of their relative abundance (%). This is an Excel file listing all 320 OTUs, abundance and the taxonomic assignment of each OTU per individual S1, S2 and S3. (XLS 94 KB) Additional file 4: Full list and relative abundance of higher taxa per individual microbiome. This is an Excel file listing all 112 higher taxa (genera or more inclusive taxa when sequences could not be confidently classified to the genus level) and their relative abundance in oral microbiomes of three individuals: S1, S2 and S3. (XLS 42 KB) Additional file 5: Relative abundance of 1660 unique sequences that were shared by three individuals (S1, S2 and S3). This Excel file lists Alpelisib datasheet the taxonomy of the sequences shared by three individuals, ranked by the abundance of these sequences in the total data set. The sequences are available at the Short Read Archive

of NCBI as SRP000913. (XLS 3 MB) Additional file 6: Full list and YM155 purchase absolute abundance of higher taxa per individual sampling site. This is an Excel file listing all 112 higher taxa (genera or

more inclusive taxa when sequences could not be confidently classified to the genus level) and their abundance in 29 samples from three individuals: S1, S2 and S3. Janus kinase (JAK) Data were not normalized. (XLS 54 KB) Additional file 7: Full list of taxa and PCA loadings. This is an Excel file listing the loadings of the first three components of the Principal Component Analysis (PCA) on all 818 OTUs (3% genetic difference) and all 29 samples (the corresponding PCA plots are shown in Figure 7). The loadings marked in bold and highlighted are above the arbitrary significance threshold of 1 or -1. The positive values are highlighted yellow; the negative values are highlighted turquoise. (XLS 128 KB) References 1. Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, Sogin ML, Jones WJ, Roe BA, Affourtit JP, Egholm M, Henrissat B, Heath AC, Knight R, Gordon JI: A core gut microbiome in obese and lean twins. Nature 2009, 457:480–484.CrossRefPubMed 2. Wilson M: Bacteriology of Humans: An Ecological Perspective. Malden, MA: Blackwell Publishing Ltd 2008. 3. Voelkerding KV, Dames SA, Durtschi JD: Next-generation sequencing: from basic research to diagnostics. Clin Chem 2009, 55:641–658.CrossRefPubMed 4. Keijser BJF, Zaura E, Huse SM, van der Vossen JMBM, Schuren FHJ, Montijn RC, ten Cate JM, Crielaard W: Pyrosequencing analysis of the oral microflora of healthy adults. J Dent Res 2008, 87:1016–1020.CrossRefPubMed 5.