Methods Savolitinib purchase Bacterial strains The following bacterial strains were tested: Staphylococcus aureus (ATCC 6538); Enterobacter aerogenes (ATCC 13048); Pseudomonas aeruginosa (ATCC 15442); Methicillin resistant Staphylococcus aureus (MRSA)(ATCC 33592); and Escherichia coli 0157:H7 (ATCC

35150). Materials The studied countertops were composed of homogenous blends of polyester, acrylic alloys and fillers, inert pigment and dyes, with (test samples) or without (control samples) Cupron’s 16% copper (I) oxide weight/weight. Three and two separate manufacturing lots of the test and control countertop samples were tested, respectively. A total of 1500 pieces, cut into one inch by one inch squares (Figure 1), 300 per each manufacturing lot, were tested. The countertops were examined by Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray spectroscopy

(EDS) by using Hitachi FE-SEM SU-70. The Cupron Enhanced EOS Surface is a novel polymeric solid surface that has all the properties of a solid surface including hardness, firmness, and the ability to be easily cleaned and shaped or fashioned with the antimicrobial ability of copper. The surface can be easily refinished and repaired in the event of damage or aesthetic appeal. find more The surfaces are currently available in two color choices due to the addition of pigments to alter the color of the surfaces at the time of manufacture. The surface is produced by Isotretinoin mixing a blend of acrylic and polyester resins with copper oxide and pigments, which is then heated until liquified and poured into casting molds. The material is allowed to cure allowing the polymerization of the material to produce a solid surface which can then be cut and shaped to produce a final product or installed surface. Figure 1 SEM pictures and EDS analysis of a representative countertop containing copper oxide particles. A. A representative picture of a tested countertop impregnated with copper oxide; B. A SEM imaging of the Countertop (white dots indicating copper oxide particles; C. EDS imaging of the Countertop (purple

dots indicating the copper oxide); D. cut through SEM imaging of the Countertop (white dots indicating copper oxide particles); and E. corresponding EDS spectra of D, showing a peak corresponding to copper. Biocidal testing protocols The biocidal testing of the countertops was buy AZD0156 conducted by an independent laboratory, MicroBiotest, a division of Microbac Laboratories, Inc. Sterling, VA, using Good Laboratory Practice (GLP) according to protocols pre-approved by the USA EPA. Protocol 1- sanitizer activity The carriers were cleaned with 70% isopropyl alcohol, rinsed with deionized water, and allowed to air dry. After steam sterilization for 15 minutes at 121°C, each carrier was placed into a plastic Petri dish matted with two pieces of filter paper using sterile forceps.