This sequence is considered to be specific to DT104 strains [4]. Positive and negative control strains were used for this marker. Of the 59 confirmed DT104 strains, all but four were positive. Furthermore, the sequence was not detected in the atypical #GSK872 concentration randurls[1|1|,|CHEM1|]# DT146 (n = 1), DT120 (n = 1), DT135 (n = 1), DT99 (n = 1), DT8 (n = 2), DT193 (n = 4), DT30 (n = 3), DT12 (n = 2), DT4 variant (n = 1), U302 (n = 12), DT2 (n = 1), DT208 (n = 1), DT12a (n = 1), DT18 (n = 1), DT36 (n = 1) or U311 (n = 1) strains.

However, we observe a cross-reaction with one DT136 strain and nine of the ten DT120 strains investigated out of the 102 strains tested. The specificity and sensitivity values for this gene target were of 89.5% and 84.6% respectively. The DT104 marker was detected in 47% of the 538 tested strains with unequal distribution among isolate sources. This marker was carried by 71% of human strains (Table 4). Furthermore, the DT104 marker was observed in around 60% of environmental samples. Nearly half the food product strains carried this marker, while the lowest frequencies occurred in poultry and other animal species, with around 40% of positive strains. – Antimicrobial resistance determinants Beta-lactam resistance including ESBL and non-ESBL producing strains was explored by targeting a family of bla TEM genes encoding TEM beta-lactamase enzymes. Reference positive strains carrying bla TEM-1, bla TEM-20, bla TEM-52 and bla TEM-63

were correctly detected with the GeneDisc® array. The bla TEM determinant was unequally click here distributed among the tested strains. The highest level–36%–was detected in human isolates. In animal or food sources, it was found in around 10 to 20% of strains (Table 4). Sulfonamide resistance was detected

by targeting the sul1 determinant, most often associated with the SGI1 gene cluster and phage type DT104 strains. sul1 rates varied according to isolation sources, the highest levels being found in swine (75%) and bovine (74%) isolates and the lowest in poultry (41%) and other minor animal species (47%). Assignment Exoribonuclease of Typhimurium genotypes All the strains were classified according to their genotype determined by the combination of the ten investigated markers. Using this combination of markers, the 538 strains were grouped into 34 different genotypes according to the UPGMA method. A dendrogram was generated using the Dice correlation coefficient. Genotypes were clustered into three main groups and two minor groups named A to E (Figure 1 and Table 2). Figure 1 Genotype constructed with the Unweighted Pair Group Method using arithmetic Averages (UPGMA) on total investigated strains with strain distribution in the main isolation sources: poultry, pigs and human sources. A black box indicates the presence of the genotype’s determinant gene. SGI1 LJ means “”SGI1 Left Junction”". Group A was composed of 211 strains divided into nine profiles: A1 to A9.