Acetylation

Acetylation selleck chemicals EPZ-5676 of tubulin by panobi nostat is consistent with HDAC6 inhibition because tubulin is one of the important substrates of HDAC6. Interestingly, the combination also enhanced the acetyl ation of histone and tubulin synergistically in Caki 1 and ACHN cells. In 769 P cells, the combination enhanced the acetylation of tubulin but not that of histone. Histone acetylation was a consequence of ubiquitinated protein accumulation We then investigated the relationship between histone acetylation and ubiquitinated protein accumulation. Pano binostat caused histone acetylation in a dose dependent fashion in all the cell lines but did not induce ubiquiti nated protein accumulation. Bortezomib, on the other hand, caused both ubiquitinated protein accu mulation and histone acetylation in a dose dependent fashion in Caki 1 and ACHN cells but did not cause histone acetylation in 769 P cells.

This is in accordance with the result that the combination did not enhance histone acetylation in 769 P cells despite inducing ubiquitinated protein accumulation in them. We inferred from these results that the histone acetylation the combination caused in Caki 1 and ACHN cells was a consequence of ubiquitinated protein accumulation. Discussion Inducing ER stress and ubiquitinated protein accumula tion is a novel approach to cancer therapy. The combin ation of an HDAC inhibitor and bortezomib is one of the combinations that might be expected to do it. The combination of panobinostat and bortezomib has recently been investigated mainly in hematological malignancies.

It has been reported that the combination of bortezomib and the HDAC inhibitor suberoylanilide hydroxamic acid inhibits renal cancer growth by causing accumulation of ubiquitinated proteins and histone acetyl ation, but that study did not show the relationship between ubiquitinated protein accumulation and histone acetylation. In the present study, using panobinostat, a more potent HDAC inhibitor, we investigated the effect of the bortezomib panobinostat combination on renal cancer growth as well as further mechanisms of the combination of bortezomib and an HDAC inhibitor. Inhibition of HDAC6 acetylates HSP90, abrogating its function and increasing the amount of unfolded proteins. We think that bortezomib inhibits degradation of unfolded proteins increased by panobinostat, which in duces ER stress and ubiquitinated protein accumulation. Accumulation of unfolded proteins, or ER stress, acti vates a signaling pathway known as the unfolded protein response, which leads to increased transcription of ER folding and quality control factors. In the present study we showed Anacetrapib the induction of ER stress by detecting the increased expression of UPR related pro teins GRP78, HSP70, Ero1 L, and ERp44.

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