At the same time, operative and long-term survival is lower in diabetic patients, suggesting increased cardiovascular burden. The higher BMS-345541 datasheet mortality in diabetics raises the question as to whether AAA repair should be individualized in selected diabetic populations at higher AAA rupture risk. (C) 2013 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.”
“BACKGROUND: As a new protein expression and self-immobilization system, cell-surface displayed enzymes

have attracted increasing attention. In this study, Geotrichum sp. lipase (GSL), an important enzyme for the enrichment of polyunsaturated fatty acids (PUFAs), was first displayed on the cell surface of

Saccharomyces cerevisiae.

RESULTS: The activity of displayed GSL was higher (43.7 U g-1 dry cell) than PARP inhibitor that of Candida antarctica lipase B (26.26 U g-1 dry cell) and that of Rhizopus oryzae lipase (4.1 U g-1 dry cell). It also exhibited higher thermostability than the free lipase, and retained 89% of the original activity after incubation at 40. C for 3 h, compared with 48% at 35. C for the free lipase at pH 8.5. Interestingly, the displayed lipase had a wider pH range and better pH stability. It had higher activity at all pH values than the free GSL, and retained 86% of the original activity in the pH range 9.5 to 10.5, whereas the activity of the free GSL could not be detected at pH 10.

CONCLUSION:

This work presented amethod to prepare a whole-cell biocatalyst with better stability and AP26113 manufacturer broader pH tolerance which will provide a useful strategy for other cost-effective self-immobilized industrial lipases. (C) 2011 Society of Chemical Industry”
“BACKGROUND: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] co-polymer has immense potential in the field of environmental and biomedical sciences as biodegradable and biocompatible material. The present study examines a filamentous N2-fixing cyanobacterium, Nostoc muscorum Agardh as a potent feedstock for P(3HB-co-3HV) co-polymer production and characterization of co-polymer film for commercial applications.

RESULTS: Under photoautotrophic growth conditions, N. muscorum Agardh accumulated the homopolymer of poly-hydroxybutyrate (PHB), whereas synthesis of P(3HB-co-3HV) co-polymer was detected under propionate-and valeratesupplemented conditions. Exogenous carbons such as acetate, fructose and glucose supplementation with propionate/valerate was found highly stimulatory for the co-polymer accumulation; the content reached 58-60% of dry cell weight (dcw) under P-/N-deficiencies with 0.4% acetate + 0.4% valerate supplementation, the highest value reported so far for P(3HB-co-3HV) co-polymer-producing cyanobacterial species.