Figure selleck chemical 2 Scanning electron micrograph of H. phototrophica DFL-43T The utilization of carbon compounds by H. phototrophica DFL-43T was also determined for this study using PM01 microplates in an OmniLog phenotyping device (BIOLOG Inc., Hayward, CA, USA). The microplates were inoculated at 28��C with a cell suspension at a cell density of 85% Turbidity and dye D. Further additives were artificial sea salts, vitamins, trace elements and NaHCO3. The exported measurement data were further analyzed with the opm package for R , using its functionality for statistically estimating parameters from the respiration curves such as the maximum height, and automatically translating these values into negative, ambiguous, and positive reactions.
The strain was studied in two independent biological replicates, and reactions with a different behavior between the two repetitions were regarded as ambiguous and are not listed below. H. phototrophica DFL-43T was positive for D,L-malic acid, D-cellobiose, D-fructose, D-galactonic acid-��-lactone, D-galactose, D-galacturonic acid, D-gluconic acid, D-glucuronic acid, D-malic acid, D-mannitol, D-melibiose, D-sorbitol, D-trehalose, D-xylose, L-alanine, L-arabinose, L-glutamic acid, L-glutamine, L-lactic acid, L-lyxose, L-malic acid, L-proline, L-serine, acetic acid, adonitol, ��-D-glucose, ��-keto-glutaric acid, ��-methyl-D-galactoside, ��-methyl-D-glucoside, bromo-succinic acid, citric acid, ethanolamine, fumaric acid, m-inositol, maltose, maltotriose, mono-methyl succinate, propionic acid, pyruvic acid, succinic acid, sucrose and uridine.
The strain was negative for 1,2-propanediol, 2′-deoxy-adenosine, D,L-��-glycerol-phosphate, D-alanine, D-aspartic acid, D-fructose-6-phosphate, D-glucosaminic acid, D-glucose-1-phosphate, D-glucose-6-phosphate, D-mannose, D-psicose, D-serine, D-threonine, L-alanyl-glycine, L-aspartic acid, L-fucose, L-galactonic acid-��-lactone, L-rhamnose, L-threonine, N-acetyl-D-glucosamine, N-acetyl-��-D-mannosamine, acetoacetic acid, adenosine, ��-D-lactose, ��-hydroxy-butyric acid, ��-hydroxy-glutaric acid-��-lactone, ��-keto-butyric acid, ��-phenylethylamine, dulcitol, glycolic acid, glycyl-L-aspartic acid, glyoxylic acid, inosine, m-hydroxy-phenylacetic acid, m-tartaric acid, mucic acid, thymidine, tricarballylic acid, tween 40, tween 80 and tyramine.
Chemotaxonomy Phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine were the GSK-3 predominant polar lipids of the membrane. The most frequent cellular fatty acids in strain DFL-43T are the mono-unsaturated straight chain acids C18:1 ��7 (62.8%) and its methylated form C18:1 ��7 11Me (21%), followed by C16:0 (6.3%) and C19:1 (3.4%) . The absorption spectrum of an acetone/methanol extract showed the presence of bacteriochlorophyll a and an additional carotenoid (possibly spheroidenone) in small amounts .