In the EtOAc and n butanol fractions, we obtained ten and three compounds respectively. Struc tural identification of those compounds was accomplished by comparison of their physical information spectral data with those reported in literature. They belong to flavones and luteolin 7 O B D glucopyranoside flavonols, quercetin, rutin, and isoquercitrin phenylethanoid glycosides, acteoside, and isoacteoside alkylated glycosides B D glucopyranosyl]oct 1 en 3 ol and 3 O oct 1 en three ol, and steroids and stigmasterol, The structures of those compounds are shown in Figure three. These compounds represent sub stances isolated from leaves created by indirect shoot organogenesis from leaf explants of H. pogonocalyx for the first time. Excluding the steroids and, each in the isolated constituents was examined separately at a relatively high concentration for anti melanogenic and neurocytoprotective activities.
Cytotoxicity and anti melanogenic activity of isolated constituents from H. pogonocalyx in HEMn cells The isolated constituents from H. pogonocalyx had been fur ther evaluated for anti melanogenic activity. Employing the MTT assay, cells have been exposed to 11 test samples, and all cells exhibited greater than 85% viability after a 24 h remedy, demonstrating that the isolated compounds exhibited no or little cytotoxicity in HEMn cells. Afterward, inhibitor price the 11 test compounds have been then ex amined for cellular anti tyrosinase activity. Acteoside exhibited higher anti tyrosinase activity than the posi tive control arbutin, and luteolin 7 O B D glucopyrano side, isoacteoside, and rutin displayed anti tyrosinase activity, Cytotoxicity and neurocytoprotective activity of isolated constituents from H. pogonocalyx in NGF differentiated PC12 cells The NGF differentiated PC12 cells have been utilized as a model to assess neurocytoprotective activity inside the present study.
In our previous study, we identified that when NGF differentiated PC12 cells were treated with 175 uM 6 OHDA for 24 h, cell viability decreased to 50. 0 4. 6% compared with that in the untreated cells, As a result, inhibitor Sorafenib we made use of 175 uM 6 OHDA to induce cytotoxicity inside the subsequent experiment. Employing the WST eight assay to evaluate cytotoxicity, PC12 cells have been exposed to the test samples, and all cells ex hibited greater than 90% viability following 24 h of treat ment, which indicated that the isolated compounds didn’t induce PC12 cell cytotoxicity, The NGF differentiated PC12 cells had been incubated with all the test compounds prior to 6 OHDA exposure, and luteolin 7 O B D glucopyranoside exhibited potent neurocytoprotective activity. Luteolin 7 O B D glucuro nide, myricetin, and rutin exhibited slightly protective activities, Discussion H. pogonocalyx is often a uncommon endemic species in Taiwan. The extract of H. pogonocalyx exhibited the totally free radical scav enging activities in our earlier study, Plant tissue culture is normally implemented for plant propagation.