Genius Pathways Analysis is aweb based application that permits visualization, exploration and exploration of related functional interactions important to the experimental results. The investigation settings employed were: Reference set: Ingenuity Knowledge Base, Relationship to include: Direct and Indirect, Includes order Clindamycin Endogenous Chemicals, Filter Summary: Consider all substances and/or connections. The most significant groups related to the uploaded datasets were identified by calculating the associated importance statistically evaluated by the Fischers exact test. The p value measures the likelihood that the association between the genes/ proteins in each Canonical Pathway and the datasets, Biological Function, etc., is not due to random chance alone determining important over representation of elements in association to certain process. We employed a p value threshold of 0. 05, limiting the false discovery rate to significantly less than 500. 100 uL of a mixture of ethanol/water 80:20 were added to 20?106 cell pellets. Cells were sonicated for 20 min and then samples were centrifuged. Supernatants were analyzed by an LC MS/MS system composed of a Alliance HT 2795 Urogenital pelvic malignancy HPLC Separation Module coupled to a Quattro Ultima Pt ESI tandem quadrupole mass spectrometer. The instrument was operated in negative electrospray ionization function usingMassLynx v. 4. 0 software and data processing was done using QuanLynx software. For HPLC evaluation, the Atlantis HILIC Silica 3 um 2. 1?150mm line was used. 25 ul of the extracted samples were injected onto the HPLC MS/MS system. The cellular phase comprised a solvent system: ACN and water containing 50mmol/l Ammoniumacetate. The first solvent composition was hundreds of A. 100%A wasmaintained for 3min, decreasing from the first natural product libraries conditions to 50%Awithin 8. 0min, holding for 4min before returning to the original state at 12. 0 min, allowing 4 min for column reequilibration. The sum total work time was 16 min, treatment toinjection. The flowrate was 0. 3ml/min. Themass spectrometer ionization source controls were optimized for maximum precursor ion yields for eachmetabolite. This was accomplished by infusing a 1 ug/mlmethanolic solution of every individual substance. These transitions were watched for the metabolites of interest: glucose 6?phosphate 258. 80 96. 80, cone 40 V and impact energy 13 eV, fructose 1,6?bisphosphate 338. 90 96. 90, cone 40 V and collision power 15 eV, glyceraldehyde 3?phosphate 168. 80 96. 90, cone 40 V and collision energy 5 eV, pyruvate 86. 90 43. 10, cone 40 V and impact energy 5 eV, lactate 88. 90 43. 10, cone 40 V and collision energy 6 eV. The voltage was 3. 00 kV, source temperature was 100 C, desolvation temperature was 400 C, and the collision cell gas pressure was 3. 5?103mbar argon.