It is also used as tonifiant.3 The bark of the plant is used to produce rinses or enemas for loin pains or kidney problems. Moreover, antibacterial and anti-yeasts activities of C. edulis extracts have been shown in previous studies.4,5 To the best of our knowledge, there is not a published report concerning the antidermatophytic activity of this plant. This study, therefore, was undertaken to first evaluate the antidermatophytic activity of the CH2Cl2-MeOH (1:1 v/v) Inhibitors,research,lifescience,medical extract, fractions and compound isolated from the stem bark of C. edulis, and then to assess the toxicological risk of its extract upon consumption.

Materials and methods General Experimental Procedures for Structure Elucidations Melting points (uncorr) were determined on a Kofler apparatus. Infra-red (IR) spectra were recorded using a Shimadzu FTIR-8400S spectrophotometer. Ultra-violet (UV) spectra were measured with a UV-210 PC, UV-Vis scanning spectrophotometer (Analytikjena). Proton Nuclear magnetic resonance (1H-NMR) spectra were Inhibitors,research,lifescience,medical recorded in CDCl3 using a Bruker Avance 500 MHz NMR spectrometer and Trimethylsilyl (TMS) as an internal standard. Column chromatography was run on Merck silica gel 60. Thin layer chromatography (TLC) were carried out either on silica gel GF254 pre-coated plates (analytical TLC) or on silica gel 60 PF254 containing gypsum (preparative

TLC), with detection accomplished Inhibitors,research,lifescience,medical by spraying with 50% H2SO4 followed by heating at 100°C, or by visualizing with a UV lamp at 254 and 366 nm. Gas chromatography-mass spectrometry (GC-MS) data were obtained with an Agilent 6890N

Network GC system/5975 Inhibitors,research,lifescience,medical Inert×L Mass selective Detector at 70 eV and 20°C. The GC column was a CP-Sil 8 CB LB, fused silica capillary column ( x , film http://www.selleckchem.com/products/Adrucil(Fluorouracil).html thickness 0.25 µm). The initial temperature was 50°C for 1 min, and was heated at 10°C/min to 300°C. The fatty acid samples of 0.5 µl were injected. The split ratio was 50:1. The carrier gas was helium at a flow rate of 1.2 ml/min. Plant Material The stem bark of C. edulis was collected from Buea (South-West Region of Cameroon) in January 2008. Inhibitors,research,lifescience,medical The plant material was identified at the Cameroon National Herbarium in Yaoundé where a voucher specimen (19357/HNC) was conserved. The plant material Sclareol was air-dried at room temperature. The dried plant material was ground into a fine powder. Extraction, Fractionation and Isolation Previously dried and powdered stem bark of C. edulis () was extracted with dichloromethane-methanol (1:1) () for 48 hours. The filtrate was concentrated under reduced pressure at 40°C using rotary vacuum evaporator to give a brown paste crude extract (). One hundred and four grams () of this extract was then subjected to fractionation as previously described.4 Briefly, the crude extract was subjected to a column chromatography with silica gel 40 (particle size 0.2-) as stationary phase.