Mej,irepresents the difference between the unknown objects selleckchem and the validation candidate dataset. When Mej,i = 0, i is the predicted variable. If the number of the predicted variable is less than cnB, then we will use Mej,i = 1 from step (2.2). According to Mej,i for every ej we choose a set of W objects; however for each object chosen there are different values ofej. For each object and based on the sets we generate a series of four filters F1 to F4. The four filter conditions are defined in steps (2.3) to (2.5).2.3. Materials2.3.1. Animal Housing and Measurement of Serum Protein Concentrations The animal housing conditions and the methods for measuring serum protein markers were described by Liou et al. [17]. Briefly, three batches of TRFCCs, batch A(nA = 76), batch B (nB = 77), and batch C (nC = 60) were included in this study.

Table 4 is the basic statistics analysis of serum protein concentrations for A, B, and C datasets. The average egg numbers for A, B, and C datasets were 94.57, 103.91, and 85.1, respectively. There were three datasets taken from three batches of birds. The birds in each batch were raised in different seasons and in different years. Total egg numbers were recorded individually and daily from 25wks to 48wks of age. Sera were collected from chickens at 14 and 24 weeks of age from batches A and B. In batch C, the sera were not collected at the same time as batch A and B. Sera for batch C were collected from chickens at 8, 14, and 22 weeks of age.

The variables, measured at 8wks and 14wks of age, were the serum protein concentrations of apolipoprotein A-I, apo VLDL-II and the X protein; the concentration of vitellogenin was also included at other time stages. Previous reports showed that these proteins participate in egg formation [12, 18]. Vitellogenin and apo A-I are major components of yolk [19, 20]. Apo VLDL-II, a lipoprotein lipase inhibitor, plays an important role in VLDL transportation from the liver to the oocyte through the plasma [21]. X protein, an IGF-I-like protein, is associated with egg production [17]. Total egg number per chicken was served as the validation variable.Table 4A basic statistical analysis for the serum protein concentrations for the A, B, and C dataset.Tables Tables5,5, ,6,6, and and77 are Pearson’s correlation Anacetrapib coefficient for A, B, and C datasets between all serum proteins, respectively. These tables show a low correlation between the number of eggs and all of the serum proteins. There is also a low correlation between each of the serum proteins, except in dataset A when the chickens were 24 weeks old.