MIRU-VNTR typing One hundred and forty seven Map isolates were typed by MIRU-VNTR and 23 different types were obtained (Table 1 and see supplementary dataset in Additional file 1). In addition, MIRU-VNTR types INMV 23 and 28 were https://www.selleckchem.com/products/bay80-6946.html obtained for the two IS901 positive M. avium isolates. The following MIRU-VNTR types were exhibited by Map isolates in this study: INMV 1 (n = 75); 2 (n = 35); 26 (n = 9); 6 (n = 4), 37

(n = 3), 8, 25, 35 (n = 2); 5, 13, 19, 20, 21, 22, 24, 27, 29, 30, 31, 32, 36, 38, 69 (n = 1). INMV 1 and 2 were the most widely disseminated MIRU-VNTR types, both occurring in the Czech Republic, Finland, The Netherlands, Scotland and Spain (Table 1 and see supplementary dataset in Additional file 1 and Additional file 2: Table S1). INMV

1 also was found in Norway and INMV 2 in Greece (Table 1 and see supplementary dataset in Additional INCB024360 purchase file 1 and Additional file 2: Table S1). The relative frequencies of the various alleles were calculated and are shown in Table 2. The allelic diversity observed is consistent with the previous report [22]. Table 2 MIRU-VNTR Allelic diversity among the Map isolates. No. of isolates with specified MIRU copy No. Locus 0 1 2 3 4 5 6 7 8 9 10 Allelic diversity (h) 292     14 47 80 3 2   1     0.58 10   21 126                 0.24 7   10 128 9               0.22 3 10 6 131                 0.2 25   2   138   7           0.1 X3   6 139 2               0.09

47     1 142 4             0.06 32                 146 1   0.006 The allelic diversity (h) at a locus was calculated as h = 1 – Σx i 2 [n/(n - 1)], where x i is the frequency of the ith allele at the locus, and n the number of isolates [52, 53]. Comparison of typing techniques A predominance of one or two types was observed with all of the typing techniques clonidine and these predominant types could be further discriminated by one or both of the other typing methods (Table 3). For example, the predominant PFGE multiplex type [2-1] comprising 83 isolates was subdivided into nine different profiles by MIRU-VNTR and seven different profiles by BstEII IS900-RFLP. PFGE multiplex type [1-1] comprising 15 isolates could be subdivided into three INMV profiles and three BstEII IS900-RFLP patterns. Minor multiplex profiles [2-30], [29-15] and [34-22] were each subdivided into two by MIRU-VNTR. The major MIRU-VNTR type INMV1 consisting of 75 isolates was split by PFGE into 11 and by BstEII IS900-RFLP into four subtypes. INMV2 composed of 35 isolates was subdivided into eight and seven types by PFGE and BstEII IS900-RFLP, respectively. The minor groups INMV 6, 8, 25, 26 and 35 were each subdivided by PFGE into a further two types and INMV 25 into two BstEII types. Both PFGE and MIRU-VNTR each differentiated the most widespread BstEII IS900-RFLP type C1, which included 71 isolates, into 14 and 11 distinct types, respectively.