Results Biofilm All sixty ST1 isolates tested were able to produce biofilm on inert surfaces. The majority (58.3% and 25%; respectively) exhibited a moderate (BU varying from 0.468 to 0.901) or strong (BU varying from 1.008 to 3.615) biofilm phenotypes (Figure 1, top). For 19 randomly selected isolates, the ability to accumulate biofilm on human Fn-coated PLX-4720 cost surfaces increased significantly (p<0.01 to p<0.0001) when compared with that on inert surfaces (Figure 1, bottom). Figure 1 Biofilm

formed by ST1 isolates. Top: Percentage of the total 60 ST1 isolates displaying strong, moderate and weak biofilm phenotypes. Wells show the different biofilm phenotypes formed on inert polystyrene surfaces by representative ST1 isolates. Bottom: Biofilm formed on inert or fibronectin-coated surfaces by 19 ST1 isolates. Proteinaceous nature of the biofilm Treatment with proteinase

K virtually disrupted preformed biofilms RAD001 in vivo for 12 ST1 isolates GKT137831 price tested. However, the carbohydrate oxidant metaperiodate almost did not affect the biofilm accumulated by these isolates (Figure 2, top). CLSM studies revealed that the agr-dysfunctional 08–008 accumulated a denser and compact biofilm when compared to the heterogeneous film formed by the agr-functional isolate (96/05). Despite the stronger biofilm phenotype displayed by the isolate 08–008, proteinase K could significantly remove the biological film accumulated (Figure 2, bottom). Figure 2 Proteinaceous nature of the biofilm. Top: Effect of 1mM/well sodium metaperiodate or 6U/well proteinase K on preformed biofilm. Wells show the effect of these compounds on biofilms preformed on inert polystyrene surfaces by representative

ST1 isolates. Bottom: Confocal laser scanning microscopy (CLSM) images of proteinase K-treated and -untreated biofilms stained with SYTO 9. The square indicates the slice of the biofilm from which the XY image was taken. The horizontal bar indicates the location of the X plane from which the cross-section was taken. Isolate 08–008 (strong biofilm producer, agr-dysfunctional), 96/05 (moderate biofilm producer, agr-functional). Role of eDNA in ST1 biofilm No correlation was detected between the activity of bacterial DNase and the levels of biofilm accumulated by 17 USA400-related isolates displaying strong, moderate or weak Unoprostone biofilm phenotypes (Figure 3, top). The addition of 28U/well DNase I in the culture media did not significantly affect the biofilm formed by these ST1 isolates. However, when this concentration was increased to 56U/well, a significant (p=0.0078) reduction of 31% in biofilm accumulation was detected (BU untreated =0.91±0.1 and treated =0.63±0.078; Figure 3, left bottom). In addition, the concentration of eDNA recovered from the supernatant of the strong biofilm producer (BU=1.167 ±0.07) isolate 08–008 was 182 ng/mL, three-times higher than that determined for the weaker producer (BU=0.348±0.