We are grateful to the animal caretakers of the Central Veterinary Institute of Wageningen University for their assistance and handling of experiments with guinea pigs. “
“The global polio eradication initiative, launched in 1988 [1]

has made significant progress in the global fight against polio. The number of polio cases worldwide has decreased by more than 99.9%, from 350 000 in 1988 to 404 cases in 2013 The number of endemic countries has selleck compound decreased from over 125 in 1988 to just three – Afghanistan, Nigeria and Pakistan – by the end of 2013 and one of the three wild poliovirus serotypes (type 2) has been eradicated (last isolated in 1999) [2]. In addition, the type 3 has not been reported since November 2012. However, to complete polio eradication, the routine use of all live-attenuated oral poliovirus vaccines must be discontinued [2]. At the

same time, maintenance of high levels of population immunity is required to protect against the emergence of vaccine-derived polioviruses and to prevent future outbreaks of wild polioviruses. Global introduction of IPV instead of OPV is needed [3] and [4]. Now that wild poliovirus type 2 is eradicated and use of OPV2 should be discontinued, the Strategic Advisory Group of Experts (SAGE) on immunization of the WHO recommends that all countries should introduce at least one dose of IPV into their routine immunization program to mitigate Bioactive Compound Library chemical structure the risks associated with the withdrawal of OPV2 [2]. A major obstacle to widespread IPV introduction is that the costs per vaccine dose of IPV are currently too high for low-income countries [5] and [6]. There is also a need for safer production of inactivated poliomyelitis vaccines, to reduce the current risks associated with using wild neurovirulent strains. Local production of IPV from attenuated poliovirus strains that have a lower biosafety risk, such as Sabin strains [7], by manufacturers in low- and middle-income countries will increase availability and may also increase affordability of inactivated poliovirus vaccines in these countries. IPV based on Sabin strains (sIPV) Linifanib (ABT-869) is being developed

by several institutes [8]. In collaboration with industrial partners, the Japan Poliomyelitis Research Institute (JPRI, Tokyo, Japan) [9] and [10], has developed a combination vaccine with sIPV combined with DTaP (diphtheria, tetanus, and acellular pertussis vaccine), which has recently received marketing authorization in Japan [11]. The Institute of Medical Biology of the Chinese Academy of Medical Sciences in Kunming has performed a phase III trial with their sIPV [12]. In response to a call from the WHO for new polio vaccines [13] and [14] Intravacc (formerly part of National Institute for Public Health and the Environment (RIVM) and Netherlands Vaccine Institute (NVI)) has developed a robust and transferable production process for IPV based on Sabin strains.