The latter is caused by the increasing nuclear Larmor frequency

The latter is caused by the increasing nuclear Larmor frequency. The ENDOR lines from different nuclei, which overlap at conventional X-band, become separated at high field. The pulse ENDOR study of short-lived paramagnetic intermediates, such as spin-correlated RPs and triplet states in the photosystems, is highly important for understanding the primary steps of photosynthesis.

In RPs, the unusual out-of-phase ESE signal appears which can be used for pulse see more ENDOR detection. Although several ENDOR investigations of photosynthetic spin-correlated RPs have been reported, the lack of a simple theory of such systems complicates the interpretation of the results. Acknowledgments The authors thank the coworkers named in the references for their important contribution to this work. Financial buy Batimastat support was obtained from the Max Planck Society Cytoskeletal Signaling inhibitor and the DFG (Sfb 663, TP A7), and from Russian Foundation for Basic Research (6-04-48021a). The President of Russian Federation grant for scientific schools (HШ-551.2008.3) is also acknowledged. References Biehl R, Plato M, Möbius K (1975) General TRIPLE resonance on free radicals in solution. Determination of relative signs of isotropic hyperfine coupling constants. J Chem Phys 63:3515–3522. doi:10.​1063/​1.​431790 CrossRef Britt RD, Campbell KA, Peloquin JM, Gilchrist ML, Aznar CP, Dicus MM, Robblee J, Messinger J (2004) Recent pulsed EPR studies of the Photosystem

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CrossRefPubMed Epel B, Niklas J, Antonkine ML, Lubitz W (2006) Absolute signs of hyperfine coupling constants as determined by pulse ENDOR of polarized radical pairs. Appl Magn Reson 30:311–327CrossRef Feher G (1956) Observation of nuclear magnetic resonances via the electron spin resonance line. Phys Rev 103:834–835. doi:10.​1103/​PhysRev.​103.​834 CrossRef Flores M, Isaacson R, Abresch E, Calvo R, Lubitz W, Feher G (2007) Protein–cofactor interaction in bacterial reaction centers from Rhodobacter sphaeroides R-26: geometry of the hydrogen bonds to the primary quinone Q A •– by 1H and 2H ENDOR spectroscopy. Biophys J 82:671–682CrossRef Freed JH (1969) Theory of saturation and double resonance effects in ESR spectra. IV. Electron-nuclear triple resonance. J Chem Phys 50:2271–2272. doi:10.​1063/​1.

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