We herein test the hypothesis that thalidomide may inhibit MAP growth.
Methods: LY3023414 nmr Using the radiometric (14)CO(2) (Bactec) system we quantified growth kinetics of thalidomide (+/-), (+), and (-) and two components for thalidomide, phthalimide and 1-hydroxypiperidine-2,6-dione (HPD). We studied four MAP strains (three human isolates, ‘Ben’, ‘Dominic’, and UCF-4, and a bovine MAP isolate 19698) and three mycobacterial controls (Mycobacterium avium and bacillus Calmette-Guerin (BCG)). Growth was quantified as growth index (GI) and inhibition as percent decrease in cumulative GI (%-Delta cGI).
Results: Phthalimide had no dose-dependent inhibition on
any strain. Neither thalidomide nor HPD inhibited M. avium or BCG. MAP inhibition varied; at 64 mu g/ml, amongst human isolates, Dominic was most susceptible: thalidomide (+) = 58%-Delta cGI and HPD = 46% – Delta cGI. UCF-4 was next: thalidomide (-) = 37%-Delta cGI and HPD = 40%-Delta cGI. Ben was least susceptible: HPD = 24%-Delta cGI.
Conclusions: We have shown, in culture, the heretofore-undescribed
inhibition of MAP growth by thalidomide and its enantiomers. Phthalimide was found to have no anti-MAP activity, whereas HPD was found to inhibit MAP growth. These data are compatible with the hypothesis that thalidomide, likeother ‘anti-inflammatories’ and ‘immunomodulators’ may act, in part, as an anti-MAP antibiotic. (C) 2009 International Society for Infectious Diseases. Published by Selleck 17-AAG Elsevier Ltd. All rights reserved.”
“The molecular weight, and intrinsic viscosity of
polybenzimidazole (PBI) and its phosphonylated derivatives are reported. The relationship between intrinsic viscosity [eta] and weight average molecular weight (M(w)) for PBI has been established in H(2)SO(4) and DMF-LiCl. The Mark Houwink constants K(w) of 5.2 x 10(-3) mL/g, alpha of 0.92 for H(2)SO(4) Solvent systems and, K(w) of 3.2 x 10(-2) mL/g, alpha of 0.754 for DMF-LiCl solvent system have been determined at M(w) < 65,000. The intrinsic viscosity of PBI determined https://www.selleckchem.com/products/dorsomorphin-2hcl.html by the Huggins-Kraemer method was compared with a single point method, and found that both methods fit well for PBI in relatively low concentration solvent system, giving similar to 99% accuracy. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 112: 3436-3441, 2009″
“Cerebral malaria is a complication of Plasmodium falciparum malaria and can result in various neurological manifestations. We report a rare case of cerebral malaria with vertebrobasilar stroke, presenting predominantly with signs of lateral medullary and cerebellar infarctions. We suggest that, in patients presenting with fever and a concurrent vertebrobasilar stroke, the possibility of cerebral malaria should also be considered.