Therefore,

a plant-based diet and the seamless integratio

Therefore,

a plant-based diet and the seamless integration of increased physical activity and Copanlisib cost social support to alter modern diets and lifestyles hold out the greatest hope for the solution of the obesity epidemic. Both public health and medical nutrition approaches can benefit from this integrative view of obesity. Am J Clin Nutr 2010;91(suppl):280S-3S.”
“BACKGROUND: Pulmonary edema and associated impaired oxygenation are a major reason for rejection of donor lung allografts offered for transplantation. Clearance of pulmonary edema can be upregulated by stimulation of beta-adrenergic receptors (beta ARs). Single-nucleotide polymorphisms (SNPs) in beta AR genes have functional effects in vitro and in vivo. We hypothesized that SNPs AZD7762 mw in beta AR genes would be associated with rates of utilization of donor lung allografts offered for transplantation.

METHODS: Nine hundred fifty-one organ donors were genotyped for 4 amino-acid-coding SNPs in the beta AR genes. Lung allograft utilization was compared among donors stratified by genotypes.

RESULTS: Utilization of donor lung allografts was 55% vs 35% (p = 0.02) among donors with GG vs AA/AG genotypes of the Ser49Gly SNP, 39% vs 32% (p = 0.04) with GG vs AA/AG genotype of Gly16Arg SNP and 37% vs 32% (p = 0.1) with CC vs GC/GG genotype of the Arg389Gly SNP. In the combined analysis,

donors carrying 0 or I associated genotype had a utilization rate of 33%, whereas donors carrying 2 or 3 associated genotypes had utilization rates of 44% and 58%, respectively (p = 0.008). There was a stepwise decrease in chest radiograph infiltrates and an increase in partial pressure of oxygen/fraction of inspired oxygen (PaO(2)/FIO(2)) with an increasing number of these associated genotypes.

CONCLUSION: Genetic variants in the beta AR genes among organ donors are associated with higher rates of lung allograft utilization.

The increased utilization may be related to increased clearance of pulmonary edema and improved oxygenation in donors with favorable genotypes and suggests that beta AR agonists may have a role in donor JNK-IN-8 molecular weight management. J Heart Lung Transplant 2011;30:211-7 (C) 2011 International Society for Heart and Lung Transplantation. All rights reserved.”
“To examine the applicability of DNA-programmed self-assembly to preparation of nanoparticle-supported catalysts, the authors performed the arrangement control of Au nanoparticles on powder supports (TiO(2) and glass) with this scheme. Scanning electron microscopy and atomic force microscopy observations confirmed that designed arrangement of two kinds of Au nanoparticles is possible on powder and crystal supports. Although catalytic activity of Au-particle/TiO(2) systems for CO oxidation was almost inhibited by the presence of DNA, it was successfully recovered by the oxygen plasma treatment.

The results demonstrate distinction between the pleural effusions

The results demonstrate distinction between the pleural effusions and good correlation with the fluid chemistry analysis to accurately

differentiate exudates and transudates for clinical purpose. The exudative effusions display a viscosity around 1.39 +/- 0.08 cP whereas the transudative effusion was measured at 0.89 +/- 0.09 cP, in good agreement with previous reports. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3615964]“
“Inflammatory and metabolic response is an important factor to determine clinical outcomes. However, it remains unknown in children undergoing heart transplantation (HTx). We examined the perioperative changes in the inflammatory Fludarabine supplier and metabolic response markers C-reactive protein (CRP) and prealbumin (PA) in 38 heart-transplanted children. Data obtained prior to and within one month after HTx included CRP, PA, total and differential white blood cell counts, doses of inotropes and immunosuppressants, cultures of blood and body fluids, duration of cardiopulmonary bypass (CPB), aortic cross clamp and donor heart ischemia, and days in the intensive care unit (ICU) and hospital. CRP was 32 +/- 49 mg/L before HTx, increased to 130 +/- 55 mg/L on postoperative

day 1-2, and decreased to mTOR inhibitor 21 +/- 31 mg/L by one month after HTx. PA was 0.15 +/- 0.06 g/L before HTx, decreased to 0.12 +/- 0.03 g/L on postoperative day 1-2, and then gradually increased to 0.21 +/- 0.10 g/L by one month after HTx. Postoperative CRP positively correlated with epinephrine dosage and CPB duration. PA positively correlated with age. In conclusion, inflammatory and metabolic Sotrastaurin solubility dmso response is present before HTx and acutely intensified after HTx. It may be mainly influenced by CPB duration and cardiovascular function status.”
“Positional information in developing embryos is specified by spatial gradients of transcriptional regulators. One of the classic systems for studying this is the activation of the hunchback (hb) gene in early fruit fly (Drosophila) segmentation by the maternally-derived gradient

of the Bicoid (Bcd) protein. Gene regulation is subject to intrinsic noise which can produce variable expression. This variability must be constrained in the highly reproducible and coordinated events of development. We identify means by which noise is controlled during gene expression by characterizing the dependence of hb mRNA and protein output noise on hb promoter structure and transcriptional dynamics. We use a stochastic model of the hb promoter in which the number and strength of Bcd and Hb (self-regulatory) binding sites can be varied. Model parameters are fit to data from WT embryos, the self-regulation mutant hb(14F), and lacZ reporter constructs using different portions of the hb promoter.

POC A1C correlated well with laboratory A1C regardless of time to

POC A1C correlated well with laboratory A1C regardless of time to follow-up.

Conclusion: POC A1C should be considered for diabetes screening in high-risk populations. If the screening had been performed with RPG alone, check details 38 individuals would have gone undetected. Early identification of individuals with elevated blood glucose will likely decrease the risk of long-term complications.”
“Background: Tuberculosis (TB) remains an important public health problem in Canadian

Aboriginal (First Nations and Inuit) communities. The objectives of this study were to predict future disease burden and set feasible targets for the elimination of TB in the First Nations population, using retrospective data and an epidemic model.

Methods: Reported TB incidence data (1974-2002), previously published TB meningitis data from the pre-chemotherapy era, and previous estimates of disease risk following infection were used to estimate HDAC inhibitor a trend in the annual risk of infection from 1929 to 2002, and the age-specific prevalence of infection in 2002. A state-transfer, compartmental model was then developed

to predict future disease burden. Two scenarios were simulated, with different disease risk parameters.

Results: The estimated prevalence of infection in 2002 was 20.9% in scenario 1 and 25.5% in scenario 2. Predicted incidence rates in 2015 were 16.8 per 100 000 and 11.7 per 100 000 for the two scenarios, respectively. The incidence of disease was not tower than 1 per 100 000 for either scenario in 2034, the arbitrarily chosen last year of the model.

Conclusions: The goal of eliminating TB among Aboriginal peoples in Canada is a feasible one, but will only be achieved with continued investment in programs designed to control and prevent transmission. Reactivation disease cases may occur for a number of years to come, making rapid elimination a difficult goal. (C) 2008 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“Depression is a very prevalent

mental disorder affecting 340 million people globally and is projected to become the leading cause of disability and the second leading contributor to the global selleck inhibitor burden of disease by the year 2020.

In this paper, we review the evidence published to date in order to determine whether exercise and physical activity can be used as therapeutic means for acute and chronic depression. Topics covered include the definition, classification criteria and treatment of depression, the link between beta-endorphin and exercise, the efficacy of exercise and physical activity as treatments for depression, properties of exercise stimuli used in intervention programs, as well as the efficacy of exercise and physical activity for treating depression in diseased individuals.

The presented evidence suggests that exercise and physical activity have beneficial effects on depression symptoms that are comparable to those of antidepressant treatments.

Following an updated review of RCC-related economic studies, we s

Following an updated review of RCC-related economic studies, we supplemented the costs of RCC reported in the literature with estimates from the latest US databases that capture the utilization of several newly approved targeted agents.

Method: We conducted analyses using the 1991-2007 SEER (Surveillance, Epidemiology and End Results)-Medicare and 1996-2007 Market Scan Commercial Claims and Encounter MCC950 datasheet (CCAE) and Medicare Supplemental

databases, and based our estimates on a prevalent cohort of patients with RCC or kidney cancer constructed from each database. All cost estimates were normalized to $US, year 2009 values. The incremental costing approach was applied to estimate the annual cost of RCC by treatment phases using a prevalent cohort of patients with RCC identified from the 2005 SEER-Medicare database. We used the method of extended estimation equations to estimate the impact of patients’ use of targeted therapies on the annual costs of RCC, while controlling for confounding factors such as patients’ age, sex, tumour characteristics, co-morbidity and geographic regions. The method was applied to two elderly cohorts of RCC patients identified from the SEER-Medicare and the MarketScan Medicare

Supplemental databases and a non-elderly selleck kinase inhibitor cohort of patients with RCC identified from the MarketScan CCAE database.

Results: Compared with the cost of treating an elderly, non-cancer patient in the matched sample, the average cost of treating an elderly patient with RCC was $US11 169 (95% CI 10683, 11 655) more per year, based on our

analyses of the latest SEER-Medicare data. The annual cost to treat patients with RCC who received targeted therapies was 3- to 4-fold greater than the cost to treat patients with RCC who received other SB431542 solubility dmso therapies. Results from the multivariate analysis showed that, after controlling for potential confounders, the annual medical cost was $US31 000-65000 higher for RCC patients treated with targeted therapies, with the largest increase observed among the non-elderly patients.

Conclusion: The economic burden of RCC is likely to grow with an increasing use of targeted therapies. Future research is needed to understand the impact of various forces on the economic burden of RCC, such as increased disease incidence, use of minimally invasive surgical techniques and more prevalent adoption of emerging targeted therapies.”
“The aim of this study was to modify pectin by covalent attachment of the water-insoluble ligand 4-aminothiophenol to its polymeric backbone. 4-Aminothiophenol is a ligand which is highly prone to oxidation. Therefore, this ligand allows oxidative cross-linking of pectin under mild oxidative conditions. Additionally, hydrophobization of pectin can be achieved by the mentioned modi. cation which offers certain advantages over highly hydrophilic native pectins.

Conclusion: Although postterm pregnancies were less likely to be

Conclusion: Although postterm pregnancies were less likely to be associated with obstetrical risk factors and complications such as fertility treatments, diabetes mellitus, hypertensive disorders, IUGR and PROM, postterm is an independent risk factor for perinatal mortality.”
“BACKGROUND: The production of biodiesel from activated sludge obtained from

Tuscaloosa, AL was optimized based on the yield of fatty acid methyl esters (FAMEs) using an in situ transesterification process. An orthogonal central composite response Anlotinib cell line surface design was considered to investigate the main and interaction effects of temperature, methanol to sludge ratio, and catalyst concentration.

RESULTS: The biodiesel yield can be satisfactorily described by the quadratic response surface model with R(2) of 0.836 and a statistically not significant lack of fit (p = 0.254). Coded regression coefficients, main effect plots

and surface plots indicated that maximum biodiesel yield may be obtained at 75 degrees C, 30 mL g(-1) (methanol/sludge) and 10% volume (catalyst concentration). Numerical optimization showed that at this reaction condition, a biodiesel yield of 3.78% (weight) can be obtained. Experimental verification gave Mocetinostat a biodiesel yield of 3.93 +/- 0.15% (weight) giving a model error of 7.35%. This indicates high reliability of the model.

CONCLUSIONS: The economic analysis showed that the in situ transesterification of wet activated sludge (84.5% weight moisture)

is less economical than the in situ transesterification of dried sludge (5% weight moisture). However, sensitivity analysis indicated that the process can be made more economical by reduction of water to 50% (weight). At this level of moisture, a biodiesel break-even price of around $7.00 per gallon is attainable, which is still more expensive than petroleum-based diesel (similar to-$2.95 per gallon). For the biodiesel from activated sludge to be economically competitive, a biodiesel yield of at least 10% (weight) is necessary. (C) 2010 Society of Chemical Industry”
“Background: Maternal obesity is a frequent obstetric risk factor, linked with short- and long-term consequences PD0325901 cost for mother and child, including foetal overgrowth, growth restriction and stillbirth. The mechanisms underlying these pathologies remain unknown but likely involve the placenta.

Aims: To study placental cell turnover in relation to maternal body mass index (BMI).

Methods: Term placental villous tissue was randomly sampled from 24 pregnancies, with a range of maternal BMI of 19.5-49.6. Immunohistochemistry was performed for human chorionic gonadotropin, Ki67 and M30 and image analysis used to calculate syncytiotrophoblast area and proliferative and apoptotic indices. Results were compared categorically between women of BMI 18.5-24.9 (normal), BMI 30.0-39.9 (obese classes 1 and 2) and BMI 40+ (obese class 3) and continuously against BMI; p<0.

The prepared emulgels were physically evaluated for their stabili

The prepared emulgels were physically evaluated for their stability after temperature cycle test, centrifugation and long-term shelf storage for 1 year at room temperature. The in vitro release at 37A degrees C was studied selleck screening library to define the effect of the concentration and type of the gelling agent. A comparison between the formulated emulgels and two commercially available products, CandistanA (R) and CanestenA (R) creams, was carried out to judge their efficacy and stability. The prepared emulgels exhibited non-Newtonian shear thinning behavior with little or no thixotropy. Four emulgels showed excellent stability as they demonstrated consistent rheological model under different treatment conditions.

The in vitro release test showed variation in the extent of percent drug released. The drug release from the

commercial preparation was lower than some of the prepared emulgel formulae. One formula containing combination of the two gelling agents (HPMC and Carbopol 934 P), showed excellent stability and high extent of clotrimazole selleck kinase inhibitor release was microbiologically evaluated against Candida albicans using cylinder and plate method. The selected formula showed superior antimycotic activity compared to the commercially available formulation. Further in vivo animal studies for the obtained stable formula is recommended.”
“Chronic pain is a complex experience stemming from the interrelationship among biological, psychological, social, and spiritual factors. Many chronic pain patients use religious/spiritual BTSA1 clinical trial forms of coping, such as prayer and spiritual support, to cope with their pain. This article explores empirical research that illustrates how religion/spirituality may impact the experience of pain and may help or hinder the coping process. This article also provides practical suggestions for health care professionals to aid in the exploration of spiritual issues that

may contribute to the pain experience.”
“Background: Various studies have reported discordant profiles of health-related quality of life (HRQOL) after stroke. The aims of this study, the first of its kind, were to determine the real impact of stroke on HRQOL across diverse cultures; and to compare HRQOL between stroke patients and healthy adults, and across stroke severity strata.

Methods: 100 stroke patients and 100 apparently healthy adults (AHAs) in Nigeria; as well as 103 stroke and 50 AHAs in Germany participated. Stroke severity was measured using the National Institute of Health Stroke Scale, Stroke Levity Scale and modified Rankin scale. HRQOL was evaluated using the HRQOL In Stroke Patients (HRQOLISP) measure, a holistic multiculturally-validated measure with seven therapeutically-relevant domains distributed into two spheres.

Results: Domains within the spiritual sphere were considered more important by stroke patients.

Results: The CE-based method showed high precision and reproducib

Results: The CE-based method showed high precision and reproducibility in determining fragment size (<1 bp). More genotypes were detected in mixtures of laboratory

lines and blood samples from malaria infected children, compared to gel electrophoresis. The capacity to distinguish recrudescent parasites from new infections in an anti-malarial drug trial was similar by both methods, resulting in the same outcome classification, however with more precise determination by CE.

Conclusion: The improved resolution and reproducibility of CE in fragment sizing allows for comparison of alleles between separate runs and determination of allele frequencies in a population. The more detailed characterization of individual msp1 and msp2 genotypes may contribute Selleck ALK inhibitor to improved assessments in anti-malarial drug trials and to a further understanding of the molecular epidemiology of these polymorphic P. falciparum antigens.”
“Background: Chronic kidney disease (CKD) is characterized by a high mortality rate, primarily due to cardiovascular disease. Reduced soluble TNF-like weak inducer of apoptosis (sTWEAK) levels have been related with endothelial function in CKD patients. selleck products However, there are no data on the relationship between sTWEAK

and its scavenger receptor CD163 and atherosclerotic burden in CKD.

Methods: A cross-sectional, observational study was conducted in 58 patients with CKD stages 1-3, 86 with CKD stages 4-5, 195 on dialysis and 86 healthy controls. The severity of atherosclerosis was estimated with the atherosclerosis score (AS), combining the results of ankle-brachial index and carotid ultrasound. sTWEAK and CD163 plasma concentrations were measured by ELISA.

Results: sTWEAK plasma levels were diminished and CD163 concentrations were increased in patients with CKD compared with controls (sTWEAK: median [interquartile

range] 308 pg/mL [258-378] vs. 371 pg/ML [319-455]; p<0.001; and CD 163: 1,047 ng/mL [740-1,495] vs. 540 ng/mL [319-765]; p<0.001; respectively). A weak but statistically significant association between sTWEAK or CD163 and CSF-1R inhibitor carotid intima-Media thickness (r = -0.109, p = 0.025;,r = 0.179, p<0.001;respectively) was observed. Patients with more severe atherosclerosis presented a higher reduction in sTWEAK concentrations (412 pg/mL. [302-322] vs. 368 pg/mL [351-385]; p<0.001) and a high-er increment in CD163 levels (1,182 ng/mL [1,107-1,258] vs. 826 ng/mL [733-919]; p<0.001). After multivariable analysis, only elevated sTWEAK levels were associated with reduced risk of atherosclerosis (0.34 [0.14-0.86], p = 0.02).

Conclusions: A significant reduction in sTWEAK and increment in CD163 plasma levels were observed in patients with more severe atherosclerosis. Our results indicate that sTWEAK could be a novel biomarker of atherosclerotic burden in CKD patients.

Results: A total of 9,749 1-3 days-old F1 female Anopheles mosqui

Results: A total of 9,749 1-3 days-old F1 female Anopheles mosquitoes were exposed to the insecticides. Among the pyrethroids, permethrin, 0.75% had the least knockdown effect, whilst cyfluthrin 0.15%, had the highest knock-down effect. Overall, no difference in susceptibility between An. gambiae 93.3% (95% CI: 92.5-94.1) and An.

funestus 94.5% (95% CI: 93.7-95.3) was observed when exposed to the pyrethroids. click here Similarly, there was no difference in susceptibility between the two vector species (An. gambiae = 79.1% (95% CI: 76.6-81.8) and An. funestus = 83.5% (95% CI: 80.2-86.4) when exposed to DDT. Overall susceptibility to the insecticides was between 80% and 98%, suggesting that there is some level of resistance, except for cyfluthrin 0.15%. find more The kdr PCR assay however, did not reveal any kdr mutations. The analysis also revealed only the molecular M (Mopti) form.

Conclusion: The findings in this study show

that An. gambiae and An. funestus, the main malaria vector mosquitoes in the Kassena-Nankana district are susceptible to the insecticides being used in the treatment of bed nets in the malaria control programme. There is however, the need for continuous monitoring of the pyrethroids as the efficacy is not very high.”
“Background: Recent studies have shown that urinary neutrophil gelatinase-associated lipocalin (NGAL) is rapidly up-regulated early after murine renal injury, and in patients after cardiac Surgery or patients critically ill with multiple trauma. In this study, we evaluated urinary NGAL levels as a potential biomarker of early acute kidney injury (AKI) in patients with severe traumatic brain injury (TBI).

Methods: All patients with severe TBI admitted to our neurosurgical intensive care unit from March to September 2011 were enrolled prospectively: Urinary NGAL was measured using a chemiluminescent microparticle immunoassay upon admission and at 24 and, 48 hours after TBI. The presence of AKI was defined by 1 the Acute Kidney Injury Network (AKIN) criteria.

Results: Using AKIN criteria, a total of 13 patients

were identified with AKI, an incidence of 24%. Those who subsequently developed AKI had a striking rise in urinary NGAL early after TBI and a sustained increase over the Epoxomicin chemical structure entire duration of the study. The urinary NGAL level of the AKI group was significantly higher than the group without AKI at all time points. Using a cutoff value of 53.9 ng/mL, the area under the receiver-operating characteristic curve for urinary NGAL at 48 hours was 0.876 with a sensitivity of 0.69 and specificity of 0.95.

Conclusions: Increased urinary NGAL is associated with an increased occurrence of AKI in patients with severe TBI. It is possible that urinary NGAL could provide a screening tool for AKI immediately after severe TBI, and this may in turn allow early intervention to ameliorate the adverse effects of AKI.

In addition, both the plasma protein-binding rates of ATRA and AT

In addition, both the plasma protein-binding rates of ATRA and ATPR to human plasma were higher than 90%.”
“Many fungal parasites enter plant cells by penetrating the host cell wall and, thereafter, differentiate specialized intracellular feeding structures, called haustoria, by invagination of the plant’s plasma membrane. Arabidopsis PEN gene products are known to act at the cell periphery and function in the execution of apoplastic immune SRT1720 price responses

to limit fungal entry. This response underneath fungal contact sites is tightly linked with the deposition of plant cell wall polymers, including PMR4/GSL5-dependent callose, in the paramural space, thereby producing localized wall thickenings called papillae. We show that powdery mildew fungi specifically induce the extracellular transport and entrapment of the

fusion protein GFP-PEN1 syntaxin and its interacting partner monomeric yellow fluorescent protein (mYFP)-SNAP33 within the papillary matrix. Remarkably, PMR4/GSL5 callose, GFP-PEN1, mYFP-SNAP33, and the ABC transporter GFP-PEN3 are selectively incorporated into extracellular encasements surrounding haustoria of the powdery mildew Golovinomyces orontii, suggesting that the same secretory defense responses become activated during the formation of papillae and haustorial encasements. This is consistent with a time-course analysis of the encasement process, indicating that these extracellular structures are generated

through the extension of papillae. We show that PMR4/GSL5 callose accumulation in papillae and haustorial encasements click here occurs independently of PEN1 syntaxin. We propose a model in which exosome BI 2536 in vivo biogenesis/release serves as a common transport mechanism by which the proteins PEN1 and PEN3, otherwise resident in the plasma membrane, together with membrane lipids, become stably incorporated into both pathogen-induced cell wall compartments.”
“To compare the short-term health-related quality of life (HRQL) between the two different routes of gastric tube reconstruction after minimally invasive esophagectomy (MIE).

From January 2007 to June 2009, 97 patients who underwent three-incision subtotal MIE were enrolled in this retrospective study. Among them, 49 patients followed prevertebral route and 48 patients followed retrosternal route. The questionnaires (EORTC QLQ C-30 and OES-18) were applied to assess the HRQL of the patients before and 2, 4, 12, 24 weeks after operation.

All the patients underwent operation with no mortality. No statistical difference was found in age, gender, serum albumin level, the level of growth in the esophagus, pathological diagnosis, tumor stage, operation time, blood loss or ICU stay between the two groups. The perioperative complication rate was 35.4% in retrosternal group and 32.7% in prevertebral group (P = 0.774).

Inclusion of coronary revascularization procedures among the inci

Inclusion of coronary revascularization procedures among the incident CHD events introduced heterogeneity. The SNPs were not associated with CHD in black participants, and associations varied in other US minorities.

Conclusions-Prospective analyses of white participants replicated several reported cross-sectional CHD-SNP associations. (Circ Cardiovasc Genet. 2011;4:661-672.)”
“In this study, polyethersulfone (PES)-alginate microcapsules were prepared for drug-controlled release, and vitamin

B(12) (VB(12)), rifampicin (RFP), and Pevonedistat research buy bovine serum albumin (BSA) were used as model drugs. Different micro-capsules were prepared by the variation of the crosslinking degree of alginate and the variation of the chemical components of the microcapsule membrane, including the PES and polyethylene glycol (PEG) contents. Systematic experiments were carried out to study their influences on the release profile of the model drugs. The results showed that with the increase of the crosslinking degree of the alginate, the

drug release rate increased; whereas with the increase of the PES concentration used to prepare the microcapsule membrane, the drug release rate decreased. The contents of the PEG in the microcapsule membrane also affected the drug release. This study enriched the methodology of the fabrication of the microcapsules, and the microcapsules may have a potential use for controlled release. (C) 2008 Wiley Periodicals, Inc. J selleck compound Appl Polym Sci 111: 651-657, 2009″
“P>Microarrays have demonstrated significant power for SB431542 cell line genome-wide analyses of gene expression, and recently have also revolutionized the

genetic analysis of segregating populations by genotyping thousands of loci in a single assay. Although microarray-based genotyping approaches have been successfully applied in yeast and several inbred plant species, their power has not been proven in an outcrossing species with extensive genetic diversity. Here we have developed methods for high-throughput microarray-based genotyping in such species using a pseudo-backcross progeny of 154 individuals of Populus trichocarpa and P. deltoides analyzed with long-oligonucleotide in situ-synthesized microarray probes. Our analysis resulted in high-confidence genotypes for 719 single-feature polymorphism (SFP) and 1014 gene expression marker (GEM) candidates. Using these genotypes and an established microsatellite (SSR) framework map, we produced a high-density genetic map comprising over 600 SFPs, GEMs and SSRs. The abundance of gene-based markers allowed us to localize over 35 million base pairs of previously unplaced whole-genome shotgun (WGS) scaffold sequence to putative locations in the genome of P. trichocarpa.